Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage IVM maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®

Article Snippet: We washed the collected oocytes in Sage IVM washing medium (Cooper Surgical), transferred them to Sage IVM maturation medium (Cooper Surgical) with 75 IU of FSH and 75 IU of luteinizing hormone (HMG Injection Teizo; Asuka Pharmaceutical, Tokyo, Japan), and kept them in an incubator (5% CO , 5% O ; 37°C) for 24 hours.

Techniques:

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Collected oocytes from resected ovaries and immunofluorescent staining. A, Collected immature oocytes before in vitro maturation in patient 8. B, An oocyte in meiosis II showing the first polar body after in vitro maturation in patient 8. C, Immunofluorescent staining of a mature oocyte in patient 5. D, Immunofluorescent staining of a mature oocyte in patient 13. The spindle is enclosed in a square box. E, Enlarged view of the spindle corresponding to the boxed area in (D). White scale bar indicates 50 µm. Red scale bar indicates 25 µm. Green, microtubules; blue, DAPI

Article Snippet: We washed the collected oocytes in Sage IVM washing medium (Cooper Surgical), transferred them to Sage IVM maturation medium (Cooper Surgical) with 75 IU of FSH and 75 IU of luteinizing hormone (HMG Injection Teizo; Asuka Pharmaceutical, Tokyo, Japan), and kept them in an incubator (5% CO , 5% O ; 37°C) for 24 hours.

Techniques: Staining, In Vitro

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Comparison of maturation rates in the transportation and non‐transportation groups. Non‐Transport, non‐transportation group; Bottle, bottle group; Box, box group. The bars indicate the percentages of oocytes that reached mature metaphase II as a result of in vitro maturation. Two‐sided Fisher's exact test was used for statistical analysis

Article Snippet: We washed the collected oocytes in Sage IVM washing medium (Cooper Surgical), transferred them to Sage IVM maturation medium (Cooper Surgical) with 75 IU of FSH and 75 IU of luteinizing hormone (HMG Injection Teizo; Asuka Pharmaceutical, Tokyo, Japan), and kept them in an incubator (5% CO , 5% O ; 37°C) for 24 hours.

Techniques: Comparison, In Vitro